Recombinant Human GSN

Synonyms: Gelsolin, GSN, AGEL, Actin-depolymerizing factor, ADF, Brevin

Mol. Weight: 115-125 kDa

Endotoxin: < 1 EU/µg as determined by LAL test.

Uniprot ID: P06396

Formulation: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 5% Trehalose, 0.1% Triton X-100, pH 8.0.

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in working aliquots at -20°C to -80°C for 3 months. Avoid repeated freeze/thaw cycle.