Recombinant human GTF2E2 protein
€0.00
In stock
SKU
ATGP1547
Description
GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and two of 34, 000 (TFIIE-beta). TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-BConcentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and two of 34, 000 (TFIIE-beta). TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-BConcentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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