Recombinant human HDDC3 protein
€0.00
In stock
SKU
ATGP1267
Description
HDDC3, also known as guanosine-3', 5'-bis (diphosphate) -pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn (2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3', 5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Guanosine-3'5'-bis(diphosphate)-pyrophosphohydrolase MESH1, Guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1, MESH1, ppGppConcentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
HDDC3, also known as guanosine-3', 5'-bis (diphosphate) -pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn (2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3', 5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Guanosine-3'5'-bis(diphosphate)-pyrophosphohydrolase MESH1, Guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1, MESH1, ppGppConcentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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