Recombinant human MGMT protein
€0.00
In stock
SKU
MMT0901
Description
O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.Alternative Names
O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase.Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.Alternative Names
O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase.Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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