Recombinant Human/Mouse/Rat Irisin

Synonyms: Fibronectin type III domain containing 5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat containing protein 2

Mol. Weight: 17-25 kDa

Activity: Measured by its ability to induce p38 MAPK activation in 3T3‑L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL. of Recombinant Human Irisin can effectively induce p38 MAPK activation.

Uniprot ID: Q8NAU1

Formulation: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex.

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.