Recombinant human NCE2/UBE2F protein
€0.00
In stock
SKU
ATGP1607
Description
uBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2F accepts the ubiquitin-like protein NEDD8 from the uBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
NEDD8-conjugating enzyme uBE2F, NCE2Concentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 1mM DTT, 200mM NaClStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
uBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2F accepts the ubiquitin-like protein NEDD8 from the uBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
NEDD8-conjugating enzyme uBE2F, NCE2Concentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 1mM DTT, 200mM NaClStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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