Recombinant human PKLR protein
€0.00
In stock
SKU
ATGP0714
Description
PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPKConcentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPKConcentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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