Recombinant human Purine nucleoside phosphorylase/PNP protein

Recombinant human Purine nucleoside phosphorylase/PNP protein

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In stock
SKU
ATGP0786
Catalog NumberATGP0786
Applications:
SDS-PAGE,Enzyme Activity
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Description
PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniquesAlternative Names
Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PuNPConcentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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