Recombinant human RNA polymerases I, II, and III subunit RPABC2/POLR2F protein
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In stock
SKU
ATGP2647
Description
POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
DNA-directed RNA polymerases I II and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III subunit RPABC2, Polymerase (RNA) II (DNA directed) polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6Concentration
1mg/ml (determined by BRADFORD assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
DNA-directed RNA polymerases I II and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III subunit RPABC2, Polymerase (RNA) II (DNA directed) polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6Concentration
1mg/ml (determined by BRADFORD assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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