Recombinant Human SEMA3A

Synonyms: Semaphorin III, SEMA3A, SEMAD, Hsema-I, SEMA1, SemD, Semad, coll-1, Hsema-III, Sema III

Mol. Weight: 70-75 kDa

Endotoxin: < 1 EU/µg as determined by LAL test.

Uniprot ID: Q14563

Formulation: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 10% Trehalose, 100 mM NaCl, 0.05% Tween 80, pH 8.5.

Purity: Greater than 95% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in working aliquots, store at -20°C to -80°C for 3 months. Avoid repeated freeze/thaw cycle.