Recombinant human SF3B14A/SF3B6 protein
€0.00
In stock
SKU
ATGP0920
Description
SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the u2 and u11/u12 small nuclear ribonucleoprotein complexes (u2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of u2 and u12 of the 17S u2 and the 18S u11/u12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
"Splicing factor 3b subunit 6, SF3b 14 kDa subunit, Spliceosome-associated protein 14-kDa, Splicing factor 3b subunit 6 14kDa, pre mRNA branch site protein p14, P14, SF3B14a, Ht006, CGI-110, SAP14a, SAP14, SF3B14 "Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the u2 and u11/u12 small nuclear ribonucleoprotein complexes (u2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of u2 and u12 of the 17S u2 and the 18S u11/u12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
"Splicing factor 3b subunit 6, SF3b 14 kDa subunit, Spliceosome-associated protein 14-kDa, Splicing factor 3b subunit 6 14kDa, pre mRNA branch site protein p14, P14, SF3B14a, Ht006, CGI-110, SAP14a, SAP14, SF3B14 "Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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