Recombinant human SOS1 protein
€0.00
In stock
SKU
ATGP3756
Description
SOS1, also known as son of sevenless homolog 1, is a Ras and Rac guanine nucleotide exchange factor. This protein is composed of several important domains. The CDC25 and REM domains provide the catalytic activity of SOS1 towards Ras and the histone fold DH/PH (Dbl homology and Pleckstrin homology) domains function, in tandem, to stimulate GTP/GDP exchange for Rac. Also, binding of GTP activates Ras proteins, and subsequent hydrolysis of the bound GTP to GDP and phosphate inactivates signaling by these proteins. GTP binding can be catalyzed by guanine nucleotide exchange factors for RAS, and GTP hydrolysis can be accelerated by GTPase-activating proteins (GAPs). Recombinant human SOS1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.Alternative Names
Son of sevenless homolog 1, SOS1, GF1, GGF1, GINGF, HGF, NS4Concentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 0.1M NaCl, 1mM DTT, 0.2mM MgCl2.Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
SOS1, also known as son of sevenless homolog 1, is a Ras and Rac guanine nucleotide exchange factor. This protein is composed of several important domains. The CDC25 and REM domains provide the catalytic activity of SOS1 towards Ras and the histone fold DH/PH (Dbl homology and Pleckstrin homology) domains function, in tandem, to stimulate GTP/GDP exchange for Rac. Also, binding of GTP activates Ras proteins, and subsequent hydrolysis of the bound GTP to GDP and phosphate inactivates signaling by these proteins. GTP binding can be catalyzed by guanine nucleotide exchange factors for RAS, and GTP hydrolysis can be accelerated by GTPase-activating proteins (GAPs). Recombinant human SOS1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.Alternative Names
Son of sevenless homolog 1, SOS1, GF1, GGF1, GINGF, HGF, NS4Concentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 0.1M NaCl, 1mM DTT, 0.2mM MgCl2.Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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