Recombinant Human TGFB1

Synonyms: CED, DPD1, LAP, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, TGF-beta 1, tgfbeta1, tgf-beta1, TGFβ, TGF-β, TGFβ1, TGF-β1

Mol. Weight: 16,38,55 kDa

Activity: Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.05-0.3 ng/mL.

Endotoxin: < 1 EU/µg as determined by LAL test.

Uniprot ID: P01137

Formulation: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex.

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.