Recombinant Human tPA

Synonyms: T-PA, TPA, t-plasminogen activator, Tissue plasminogen activator

Mol. Weight: 55-80 kDa

Endotoxin: < 1 EU/µg as determined by LAL test.

Uniprot ID: P00750

Formulation: Lyophilized from a 0.2 μm filtered solution of 20 mM Succinate, 4% Trehalose, 4% Mannitol, 0.2 mM CaCl2, 0.02% Tween 80, pH 4.5.

Purity: Greater than 95% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in working aliquots at -20°C to -80°C for 3 months. Avoid repeated freeze/thaw cycle.