Recombinant human UBA5 protein
€0.00
In stock
SKU
ATGP1507
Description
uBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and uBA5 subfamily. ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. uBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SuMO2. Recombinant human uBA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
Ubiquitin-like modifier-activating enzyme 5, THIFP1, uBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaClStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
uBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and uBA5 subfamily. ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. uBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SuMO2. Recombinant human uBA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
Ubiquitin-like modifier-activating enzyme 5, THIFP1, uBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaClStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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