Recombinant human Ubc12/UBE2M protein
€0.00
In stock
SKU
ATGP1603
Description
uBE2M, also known as NEDD8-conjugating enzyme ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Recombinant human uBE2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M, Homologous to yeast UBC12, Ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), hUbc12, UBC12Concentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
uBE2M, also known as NEDD8-conjugating enzyme ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Recombinant human uBE2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M, Homologous to yeast UBC12, Ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), hUbc12, UBC12Concentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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