Recombinant Human UBE2E3

Synonyms: Homologous to yeast UBC4/5, UB2E3_HUMAN, UBC E4, Ubc M2, UBC4/5 homolog, UBCE 4, UBCE4, UBCH 9, UbcH9, UbcM2, UBE2 E3, UBE2E3, Ubiquitin carrier protein, Ubiquitin carrier protein E3, Ubiquitin conjugating enzyme E2 23 kDa, Ubiquitin conjugating enzyme E2 E3

Mol. Weight: 22.7 kDa

Uniprot ID: Q969T4

Formulation: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex.

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.