Recombinant mouse Bleomycin Hydrolase/BLMH protein
€0.00
In stock
SKU
ATGP3880
Description
BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug Blm. It catalyzes the inactivation of the antitumor drug Blm (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant mouse BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.Alternative Names
Bleomycin hydrolase, BH, BLM hydrolase, BMHConcentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 30% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug Blm. It catalyzes the inactivation of the antitumor drug Blm (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant mouse BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.Alternative Names
Bleomycin hydrolase, BH, BLM hydrolase, BMHConcentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 30% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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