Recombinant mouse eIF-4E/EIF4E protein
€0.00
In stock
SKU
ATGP3550
Description
Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant mouse Eif4e protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, eIF-4F 25 kDa subunitConcentration
1mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSFStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant mouse Eif4e protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, eIF-4F 25 kDa subunitConcentration
1mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSFStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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