Recombinant mouse Neutrophil Elastase/ELA2 protein
€0.00
In stock
SKU
ATGP3873
Description
Neutrophil Elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha 1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Recombinant Mouse Neutrophil Elastase, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.Alternative Names
Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastaseConcentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Neutrophil Elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha 1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Recombinant Mouse Neutrophil Elastase, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.Alternative Names
Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastaseConcentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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