Recombinant Mouse PF4

Synonyms: C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, chemokine ligand 4, CXCL 4, CXCL4, Iroplact, Oncostatin A, Oncostatin-A

Mol. Weight: 10.1 kDa

Activity: Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 5-20 μg/mL.

Endotoxin: < 1 EU/µg as determined by LAL test.

Uniprot ID: Q9Z126

Formulation: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex.

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.