Reticulon-1C / NSP-C (clone RNL-4), anti-human, rat, swine
€564.00
In stock
SKU
ARP-21-5245
Catalog Number: 21-5245
Size: 100 µg
Isotype: Mouse IgG1
Size: 100 µg
Isotype: Mouse IgG1
Background:
Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
Clone: RNL-4
Isotype: Mouse IgG1
Source: RNL-4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells, with spleen cells from a BALB/c Mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
Reactivity: human, rat, swine
Specificity: RNL-4 recognizes an epitope loCated within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indiCate neuronal differentiation and to be downregulated in neurological pathologies.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (frozen), Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
Clone: RNL-4
Isotype: Mouse IgG1
Source: RNL-4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells, with spleen cells from a BALB/c Mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
Reactivity: human, rat, swine
Specificity: RNL-4 recognizes an epitope loCated within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indiCate neuronal differentiation and to be downregulated in neurological pathologies.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (frozen), Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
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