Reverse triiodothyronine (rT3) detection kit

Use: Used to detect the concentration of trans-triiodothyronine (rT3) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled anti-triiodothyronine (rT3) and purified anti-triiodothyronine (rT3) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibody and biotin-labeled anti-triiodothyronine (rT3) and non-labeled antigen (calibrator or specimen) inhibit the competitive reaction. The binding amount of antibody and biotin-labeled anti-triiodothyronine (rT3) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled anti-triiodothyronine (rT3), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated anti-triiodothyronine (rT3) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated anti-triiodothyronine (rT3)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of rT3 increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of rT3 in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.