RFC5 polyclonal, anti-human, mouse

RFC5 polyclonal, anti-human, mouse

€388.00
In stock
SKU
BS71063
Catalog Number: BS71063
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC

Datasheet
Background:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.

Alternative Name:
Replication factor C subunit 5, Activator 1 36 kDa subunit, A1 36 kDa subunit, Activator 1 subunit 5, RFC36

Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200

Specificity: RFC5 polyclonal antibody detects endogenous levels of RFC5 protein.

Immunogen:
Recombinant protein of human RFC5.

MW: ~ 38 kDa

Swis Prot.: P40937

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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