RhoA (phospho-Ser-188) Blocking Peptide
€155.00
In stock
SKU
ECM-RX1365
Background:
Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDP-bound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.
Background References
Lang, P. et al. (1996) EMBO J. 15:510.
Dong, J.M. et al. (1998) J. Biol. Chem. 273(35):22554.
Sawada, N. et al. (2001) Biochem. Biophys. Res. Commun. 280:798.
Ellerbroek, S.M. et al. (2003) J. Biol. Chem. 278(21):19023.
Sequence: Phospho-RhoA (Ser-188) synthetic peptide corresponds to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat and mouse RhoA, but is not found in RhoB or RhoC proteins.
Specificity: The peptide is specifically recognized by anti-RhoA (Ser-188) phospho-specific antibody (RP1361) in ELISA, and has been shown to block the reactivity of RP1361 during Western blot. In addition, the peptide is recommended for use in blocking RP1361 reactivity in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDP-bound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.
Background References
Lang, P. et al. (1996) EMBO J. 15:510.
Dong, J.M. et al. (1998) J. Biol. Chem. 273(35):22554.
Sawada, N. et al. (2001) Biochem. Biophys. Res. Commun. 280:798.
Ellerbroek, S.M. et al. (2003) J. Biol. Chem. 278(21):19023.
Sequence: Phospho-RhoA (Ser-188) synthetic peptide corresponds to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat and mouse RhoA, but is not found in RhoB or RhoC proteins.
Specificity: The peptide is specifically recognized by anti-RhoA (Ser-188) phospho-specific antibody (RP1361) in ELISA, and has been shown to block the reactivity of RP1361 during Western blot. In addition, the peptide is recommended for use in blocking RP1361 reactivity in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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