RNF40 polyclonal, anti-human

Background:
Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF40 (RING finger protein 40), also known as BRE1B, Staring or RBP95, is a 1,001 amino acid nuclear protein that contains one RING-type zinc finger. Expressed ubiquitously with highest expression in heart, testis and pancreas, RNF40 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B and Syntaxin 1. In addition, RNF40 forms a ubiquitin ligase complex with UBCH6 (an E2 enzyme) and together, these proteins play a crucial role in regulation of the histone code. Four isoforms of RNF40 exist due to alternative splicing events.

Alternative Name:
E3 ubiquitin-protein ligase BRE1B, BRE1-B, 95 kDa retinoblastoma-associated protein, RBP95, RING finger protein 40, RNF40, BRE1B, KIAA0661

Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100, ChIP: 1:50 - 1:200

Specificity: RNF40 polyclonal antibody detects endogenous levels of RNF40 protein.

Immunogen:
Recombinant full length Human RNF40.

MW: ~ 114 kDa

Swis Prot.: O75150

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.