RNP particle (clone 58-15), anti-human, rat

Source:
A BALB/c mouse was immunized with isolated nuclei.
Fusion partner: NS-1.

Specifications:
58-15 Recognizes riboncleoproteins (RNP), found predominantly in nuclear ribonucleoprotein (nRNP) particles, one of the main components of nucleoli. It identifies cells active in the cell cycle and hence can be used to measure the mitotic activity of cell populations. Since the antibody can be used in paraffin embedded tissue sections, it can identify actively cycling cells within routinely fixed tissue specimens. 58-15 Can be considered a pan nRNP antibody. Pan nRNP antibodies provide detection for a range of RNP proteins.

Species reactivity:
Positive: human, rat. Negative: mouse.

Applications:
58-15 Can be viewed as a pan nRNP antibody. Pan nRNP antibodies provide detection for a range of RNP proteins.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate/trypsin

Positive control: Jurkat cells, K-562 cells, spleen.

References:
- Clevenger, C.V. et al. J Histochem Cytochem32: 757-765 (1984).
- Clevenger, C.V. et al. Cytometry6: 208-214 (1985).
- Maryam A and Nigel WF, J Virol.75: 12070-12080, (2001).