Robo1 (C-terminal region) Blocking Peptide
€155.00
In stock
SKU
ECM-RX2795
Background:
The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.
Background References
Kidd, T. et al. (1998) Cell 92:205.
Bashaw, G.J. et al. (2000). Cell 101:703.
Yang, L. & Bashaw, G.J. (2006). Neuron 52:595.
Sequence: Robo1 synthetic peptide corresponds to amino acids in the C-terminal region of human Robo1 This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.
Specificity: This peptide is recognized by Robo1 (C-terminal region) antibody (RP2791) in ELISA, and has been shown to block the reactivity of RP2791 in Western blot, and is recommended for blocking in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.
Background References
Kidd, T. et al. (1998) Cell 92:205.
Bashaw, G.J. et al. (2000). Cell 101:703.
Yang, L. & Bashaw, G.J. (2006). Neuron 52:595.
Sequence: Robo1 synthetic peptide corresponds to amino acids in the C-terminal region of human Robo1 This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.
Specificity: This peptide is recognized by Robo1 (C-terminal region) antibody (RP2791) in ELISA, and has been shown to block the reactivity of RP2791 in Western blot, and is recommended for blocking in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
| Is Featured? | No |
|---|
Write Your Own Review