RPE65 monoclonal, anti-human, mouse, rat

Description: Anti-RPE65 Rabbit Monoclonal Antibody . Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association..

Other Names: Retinoid isomerohydrolase, 3.1.1.64, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, 5.3.3.22, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65 (HGNC:10294)

Clone Name(s):
Clone: ABAH-18

Subcellular Localization: Cytoplasm. Cell membrane ; Lipid-anchor. Microsome membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated (By similarity). Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells..

Tissue Specificity: Retinal pigment epithelium specific..

Gene ID: 6121

Immunogen: A synthesized peptide derived from human RPE65

Calculated MW: 60948

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.