SAPK/JNK (Phospho-Tyr185) polyclonal, anti-human, mouse, rat

Specificity:
SAPK/JNK (Phospho-Tyr185) Antibody detects endogenous levels of MAPK8/9/10 only when phosphorylated at Tyr185.

Synonyms: JNK-46, JNK1-alpha-2, MAPK8, MK08, Mitogen-activated protein kinase 8, PRKM8, SAPK gamma, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, kinase JNK1, p54 gamma

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185.

Immunogen Sequence:
Phospho

Modification Sites: H:Y185/223, M:Y185/223, R:Y185/223

MW (kDa): 48 kDa

NCBI Gene Symbol: MAPK8/9/10

Database Links:
Gene ID: 5599/5601/5602
Swiss Prot No.: P45983/P45984/P53779
OMIM No.: 601158/602896/602897/606369
Unigene No.: Hs.138211/Hs.654461/Hs.125503

Application Dilutions: WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.