SCAI (N-terminal region) Blocking Peptide
€155.00
In stock
SKU
ECM-SX3845
Background:
Cellular processes such as proliferation, differentiation, and cell migration are directed through the highly regulated control of transcriptional cofactors. During cell migration, serum response factor (SRF) is a central transcription factor that interacts with MAL to induce gene expression in response to actin assembly changes caused by the RhoA-mDia1 pathway. SCAI (suppressor of cancer cell invasion) is a highly conserved protein that is thought to regulate invasive cell migration and act on the RhoA–mDia1 signal transduction pathway. SCAI localizes in the nucleus, where it is thought to bind and inhibit MAL, by forming a ternary complex with SRF. Suppression of SCAI expression has been shown to upregulate β1-integrin expression, an intergrin that is also upregulated in several human malignancies. In addition, SCAI is downregulated in various human tumors, and decreased levels of SCAI correlate with increased invasive cell migration. Thus, SCAI is a transcriptional cofactor that may be important for regulating gene expression downstream of mDia1 to direct changes in cell motility.
Sequence: SCAI synthetic peptide corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.
Specificity: The peptide is specifically recognized by SCAI (N-terminal) antibody (SP3841) in ELISA, and has been shown to block the reactivity of SP3841 in Western blot. In addition, the peptide is recommended for use in blocking SP3841 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Cellular processes such as proliferation, differentiation, and cell migration are directed through the highly regulated control of transcriptional cofactors. During cell migration, serum response factor (SRF) is a central transcription factor that interacts with MAL to induce gene expression in response to actin assembly changes caused by the RhoA-mDia1 pathway. SCAI (suppressor of cancer cell invasion) is a highly conserved protein that is thought to regulate invasive cell migration and act on the RhoA–mDia1 signal transduction pathway. SCAI localizes in the nucleus, where it is thought to bind and inhibit MAL, by forming a ternary complex with SRF. Suppression of SCAI expression has been shown to upregulate β1-integrin expression, an intergrin that is also upregulated in several human malignancies. In addition, SCAI is downregulated in various human tumors, and decreased levels of SCAI correlate with increased invasive cell migration. Thus, SCAI is a transcriptional cofactor that may be important for regulating gene expression downstream of mDia1 to direct changes in cell motility.
Sequence: SCAI synthetic peptide corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.
Specificity: The peptide is specifically recognized by SCAI (N-terminal) antibody (SP3841) in ELISA, and has been shown to block the reactivity of SP3841 in Western blot. In addition, the peptide is recommended for use in blocking SP3841 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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