SCF mouse active recombinant protein
€295.00
In stock
SKU
600214
Protein Family: Cytokines
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: Kit ligand, C-kit ligand, stem cell factor, SCF, mast cell growth factor, MGF, KITLGDescription:
Recombinant mouse Stem Cell Factor (SCF) is involved in survival, differentiation and activation of myeloid, erythroid, lymphoid, mast cells, eosinophils and other progenitor cells. mSCF signals through its receptor, tyrosine kinase SCF R/c-kit. It is produced in E. coli as a single non-glycosylated polypeptide chain containing 164 amino acids with a MW of 18,309 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Mouse SCF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent stimulation of Human TF-1 cells, is 5.5 ng/ml.
Accession No.: P20826
MW: 18309 Da
Sequence:
KEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH6.5. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: Kit ligand, C-kit ligand, stem cell factor, SCF, mast cell growth factor, MGF, KITLGDescription:
Recombinant mouse Stem Cell Factor (SCF) is involved in survival, differentiation and activation of myeloid, erythroid, lymphoid, mast cells, eosinophils and other progenitor cells. mSCF signals through its receptor, tyrosine kinase SCF R/c-kit. It is produced in E. coli as a single non-glycosylated polypeptide chain containing 164 amino acids with a MW of 18,309 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Mouse SCF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent stimulation of Human TF-1 cells, is 5.5 ng/ml.
Accession No.: P20826
MW: 18309 Da
Sequence:
KEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH6.5. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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