SDF-1-alpha human active recombinant protein
€295.00
In stock
SKU
600171
Protein Family: Cytokines
Pathway and Disease: Development and behavior, Immune System, Signaling Molecules and Interaction
Alternate Names: Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Pre-B cell growth stimulating factor, PBSF, Intercrine reduced in hepatomas, IRH, hIRH, SDF-1-beta(3-72), SDF-1-alpha(3-67), CXCL12, SDF1, SDF1A, SDF1BDescription:
Stromal-Derived Factor (SDF-1) is a chemoattractant active on T-lymphocytes and monocytes, but not neutrophils. This cytokine activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-alpha (3-67) and SDF-1-beta (3-72) show a reduced chemotactic activity. Binding to cell surface proteoglycans inhibits formation of SDF-1-alpha (3-67), preserving the activity of SDF-1 on local sites. SDF-2 acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase SDF-1 stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. Recombinant Human SDF-1 produced in E. coli is a non-glycosylated polypeptide chain containing 68 amino acids with a MW of 8,008 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
SDF-1a is fully biologically active when compared to standard. The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 10 - 75 ng/ml.
Accession No.: P48061
MW: 8008 Da
Sequence:
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Development and behavior, Immune System, Signaling Molecules and Interaction
Alternate Names: Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Pre-B cell growth stimulating factor, PBSF, Intercrine reduced in hepatomas, IRH, hIRH, SDF-1-beta(3-72), SDF-1-alpha(3-67), CXCL12, SDF1, SDF1A, SDF1BDescription:
Stromal-Derived Factor (SDF-1) is a chemoattractant active on T-lymphocytes and monocytes, but not neutrophils. This cytokine activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-alpha (3-67) and SDF-1-beta (3-72) show a reduced chemotactic activity. Binding to cell surface proteoglycans inhibits formation of SDF-1-alpha (3-67), preserving the activity of SDF-1 on local sites. SDF-2 acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase SDF-1 stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. Recombinant Human SDF-1 produced in E. coli is a non-glycosylated polypeptide chain containing 68 amino acids with a MW of 8,008 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
SDF-1a is fully biologically active when compared to standard. The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 10 - 75 ng/ml.
Accession No.: P48061
MW: 8008 Da
Sequence:
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
| Is Featured? | No |
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