Secretory component (free and bound) (clone NI 194-4 (A89-039)) HRP, anti-human

Background:
The reactivity of the antiserum is restricted to free and bound secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

Clone: NI 194-4 (A89-039)

Isotype: Mouse IgG1

Source: Highly purified secretory component isolated from human milk.

Reactivity: human. The anti-serum does not react with any other component of the human milk or plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human secretory component, free and bound.

Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.