Sema-4D and Plexin B1 Receptor Antibody Sampler Kit

Background:
Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. In addition to these motifs, the Plexin B1 and B2 family members contain cleavage sites for subtilisin-like proprotein convertases. These cleavage sites may be important for regulating assembly of heterodimeric receptors or release of the extracellular domain. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D. Sema-4D activation of Plexin B1 recruits and activates c-Met, which can promote cell survival, migration, and angiogenesis.

Buffer/Storage:
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptide is supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.