Semaphorin-3A (C-terminal) Blocking Peptide
€155.00
In stock
SKU
ECM-SX1245
Background:
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Sequence: Semaphorin 3A synthetic peptide corresponding to amino acids in the C-terminal region of human Sema3A. This sequence has two amino acid differences from rat and mouse Sema3A, and has low homology to other semaphorin family members.
Specificity: The peptide is specifically recognized by anti-Sema3A (C-terminal) antibody (SP1241) in ELISA, and has been shown to block the reactivity of SP1241 during Western blot. In addition, the peptide is recommended for use in blocking SP1241 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Sequence: Semaphorin 3A synthetic peptide corresponding to amino acids in the C-terminal region of human Sema3A. This sequence has two amino acid differences from rat and mouse Sema3A, and has low homology to other semaphorin family members.
Specificity: The peptide is specifically recognized by anti-Sema3A (C-terminal) antibody (SP1241) in ELISA, and has been shown to block the reactivity of SP1241 during Western blot. In addition, the peptide is recommended for use in blocking SP1241 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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