Semaphorin-3A Recombinant Protein Lysate
€179.00
In stock
SKU
ECM-SL7441
Background:
One family of inhibitory axon guidance molecules is the semaphorins, which include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance during neuronal development and after CNS injury. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Human recombinant Semaphorin-3A protein includes amino acids 26 to 771, as well as a C-terminal human IgG FC domain. The protein is detected by rabbit polyclonal anti-Sema3A (N-terminal region) (Cat.#SP1401), anti-Sema3A (Central region) (Cat.#SP1221), and anti-Sema3A (C-terminal) (Cat.#SP1241). The antibodies detect both full length Sema3A/Fc chimera (125 kDa) and the cleaved full length Sema3A (95 kDa).
Buffer/Storage:
Sema-3A recombinant protein lysate is supplied at a concentration of 10 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
One family of inhibitory axon guidance molecules is the semaphorins, which include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance during neuronal development and after CNS injury. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Human recombinant Semaphorin-3A protein includes amino acids 26 to 771, as well as a C-terminal human IgG FC domain. The protein is detected by rabbit polyclonal anti-Sema3A (N-terminal region) (Cat.#SP1401), anti-Sema3A (Central region) (Cat.#SP1221), and anti-Sema3A (C-terminal) (Cat.#SP1241). The antibodies detect both full length Sema3A/Fc chimera (125 kDa) and the cleaved full length Sema3A (95 kDa).
Buffer/Storage:
Sema-3A recombinant protein lysate is supplied at a concentration of 10 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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