Semaphorin-3F (a.a. 746-762) Blocking Peptide
€155.00
In stock
SKU
ECM-SX3925
Background:
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3F (Sema-3F) is a class III secreted semaphorin that binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. During peripheral and central nervous system development, Sema-3F has critical roles in axon guidance and dendrite outgrowth. Sema-3F has also been shown to inhibit angiogenesis by manipulating VEGFR function and decreasing blood vessel density. In cancers, Sema-3F induces a poorly vascularized, encapsulated, non-metastatic phenotype through chemorepulsion of endothelial cells in melanoma, while Sema-3F disrupts intercellular contacts of MCF7 breast cancer cells through delocalization of E-cadherin and β-catenin. Thus, Sema-3F may have important roles in axon guidance, angiogenesis, and tumor progression.
Sequence: Semaphorin 3F synthetic peptide corresponds to amino acids 746 to 762 in human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members.
Specificity: The peptide is specifically recognized by anti-Sema-3F (a.a. 746-762) antibody (SP3921) in ELISA, and has been shown to block the reactivity of SP3921 during Western blot. In addition, the peptide is recommended for use in blocking SP3921 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3F (Sema-3F) is a class III secreted semaphorin that binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. During peripheral and central nervous system development, Sema-3F has critical roles in axon guidance and dendrite outgrowth. Sema-3F has also been shown to inhibit angiogenesis by manipulating VEGFR function and decreasing blood vessel density. In cancers, Sema-3F induces a poorly vascularized, encapsulated, non-metastatic phenotype through chemorepulsion of endothelial cells in melanoma, while Sema-3F disrupts intercellular contacts of MCF7 breast cancer cells through delocalization of E-cadherin and β-catenin. Thus, Sema-3F may have important roles in axon guidance, angiogenesis, and tumor progression.
Sequence: Semaphorin 3F synthetic peptide corresponds to amino acids 746 to 762 in human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members.
Specificity: The peptide is specifically recognized by anti-Sema-3F (a.a. 746-762) antibody (SP3921) in ELISA, and has been shown to block the reactivity of SP3921 during Western blot. In addition, the peptide is recommended for use in blocking SP3921 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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