Semaphorin-6A (C-terminus) Blocking Peptide

Background:
The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 6A (Sema-6A) is a single-pass type I membrane protein that contains a plexin-semaphorin-integrin (PSI) domain and a Sema domain. Sema-6A is active as a homodimer or oligomer. The Sema-6A homodimer interacts with a Plexin A2 homodimer. Sema-6a promotes reorganization of the actin cytoskeleton to regulate normal granule cell migration in the developing cerebellum and to control axon guidance in the developing central nervous system. In addition, Sema-6a has been implicated in oligodendrocyte myelination and may alter VEGF signaling during angiogenesis.

Sequence: Semaphorin 6A synthetic peptide corresponding to amino acids in the C-terminus of human Sema-6A. The sequence used is identical to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.

Specificity: The peptide is specifically recognized by Semaphorin-6A (C-terminus) antibody (SP5361) in ELISA, and has been shown to block the reactivity of SP5361 in Western blot. In addition, the peptide is recommended for use in blocking SP5361 reactivity in immunocytochemistry.

Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.