SF3B3 polyclonal, anti-human, mouse, rat
€305.00
In stock
SKU
BS5926
Background:
SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
Alternative Name:
Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, KIAA0017, SAP130
Application Dilution: WB: 1:500~1:1000
Specificity: SF3B3 polyclonal antibody detects endogenous levels of SF3B3 protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 282-327 of Human SF3B3.
MW: ~ 130 kDa
Swis Prot.: Q15393
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
Alternative Name:
Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, KIAA0017, SAP130
Application Dilution: WB: 1:500~1:1000
Specificity: SF3B3 polyclonal antibody detects endogenous levels of SF3B3 protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 282-327 of Human SF3B3.
MW: ~ 130 kDa
Swis Prot.: Q15393
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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