Shb (phospho-Y246) polyclonal, anti-human, mouse

Background:
The SH2 (Src Homology 2) domain is a structurally conserved motif that contains two alpha helices and seven beta strands and is found in a variety of proteins that are involved in signal transduction throughout the cell. Specifically, the SH2 domain targets SH2 domain-containing proteins to tyrosinephosphorylated sites, an event that can trigger a protein-protein interaction cascade which may ultimately effect gene expression and cellular function. Shb (SH2 domain-containing adapter protein b), Shd (SH2 domain-containing adapter protein d), She (SH2 domain-containing adapter protein e) and Shf(SH2 domain-containing adapter protein f) are SH2 domain-containing proteins that play various roles throughout the cell.

Alternative Name:
SH2 domain-containing adapter protein B, SHB

Application Dilution: IHC: 1:50~1:200

Specificity: p-Shb (Y246) polyclonal antibody detects endogenous levels of Shb protein only when phosphorylated at Tyr246.

Immunogen:
Synthetic phosphopeptide derived from human Shb around the phosphorylation site of Tyrosine 246.

MW: ~55kDa

Swis Prot.: Q15464

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.