SHP (clone SH2G5-C), anti-human

Decription:
Small heterodimer partner (SHP) is a 257-amino acid orphan nuclear receptor expressed in the liver, pancreas, spleen, small intestine, and adrenal gland in humans. SHP inhibits the transcriptional activity of hepatocyte nuclear factor-4alpha (HNF4alpha). Recently a cohort of Japanese patients with maturity onset diabetes of the young (MODY) were screened and seven mutations found that result in impaired inhibition of HNF-4 transcriptional activity in vitro. Defects in NR0B2 may be associated with early-onset obesity.

Synonyms: Small Heterodimer Partner, Orphan Nuclear Receptor SHP, Nuclear Receptor Subfamily 0 Group M member 2, NR0B2

Clone: SH2G5-C

Isotype: Rat IgG2a kappa

Immunogen: Recombinant human SHP.

Reactivity: human

Specificity: Recognizes human SHP. Detects a band of ~30kDa by Western blot.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct or indirect: 1:5'000-1:10'000)
Western blot: (1:2'000-1:10'000 using ECL).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)