sIL-2Rα monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10512
Catalog Number: AC-ABG10512
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in mice using highly pure recombinant Human soluble IL-2 Receptor alpha as the immunizing antigen. This IgG2a kappa antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human sIL-2Ralphaby Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2.0-4.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents.
Immunohistochemistry:
This antibody stained frozen human tonsil. The recommended concentration is 5.0 μg/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in mice using highly pure recombinant Human soluble IL-2 Receptor alpha as the immunizing antigen. This IgG2a kappa antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human sIL-2Ralphaby Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2.0-4.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents.
Immunohistochemistry:
This antibody stained frozen human tonsil. The recommended concentration is 5.0 μg/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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