Sirtuin 5 (clone S5R37-3), anti-human

Decription:
Sirtuin-5 is a NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during fasting. Can deacetylate cytochrome c and a number of other proteins (in vitro).

Synonyms: SIRT5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein 5, SIR2L5

Clone: S5R37-3

Isotype: Mouse IgG2b kappa

Immunogen: Recombinant human sirtuin 5.

Reactivity: human

Specificity: Recognizes human sirtuin 5. Detects a band of ~37kDa by Western blot.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct and indirect: 1:2'000-1:5'000)
Western Blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)