SLC4A2 polyclonal, anti-human

Background:
Primary canalicular bile undergoes a process of fluidization and alkalinization along the biliary tract that is influenced by several factors, including hormones, innervation/neuropeptides and biliary constituents. The excretion of bicarbonate at both the canaliculi and the bile ducts is an important contributor to the generation of bile-salt independent flow. Bicarbonate is secreted from hepatocytes and cholangiocytes through parallel mechanisms, which involve chloride efflux through activation of chloride channels and further bicarbonate secretion via AE2 (also designated SLC4A2)-mediated chloride/bicarbonate exchange. The AE2 protein regulates pH, chloride concentration, cell volume and transepithelial ion transport in many tissues. Gene silencing of AE2 causes a marked inhibition of unstimulated and secretin-stimulated chloride/bicarbonate exchange, which maintains the bile acid pool that is crucial for secretin to induce bicarbonate-rich choleresis.

Alternative Name:
AE2, HKB3, BND3L, NBND3, EPB3L1,

Application Dilution: WB: 1:500 - 1:2000

Specificity: SLC4A2 polyclonal antibody detects endogenous levels of SLC4A2 protein.

Immunogen:
Recombinant protein of human SLC4A2

MW: Predicted band size: 137 kDa, Observed band size: Refer to Figures

Swis Prot.: P04920

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.