Smad 5 (phospho-S463/465) (clone ), anti-human, mouse, rat

Smad 5 (phospho-S463/465) (clone ), anti-human, mouse, rat

€428.00
In stock
SKU
BS9889M
Catalog Number: BS9889M
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, ICC, IHC

Datasheet
Background:
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

Alternative Name:
Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5

Application Dilution: WB: 1:1000-1:2000, ICC/IHC: 1:50-1:200

Specificity: This antibody detects endogenous levels of human Smad 5 protein only when phosphorylated at Ser463 and Ser465.

Immunogen:
Recombinant antibody.

MW: ~ 58 kDa

Swis Prot.: Q99717

Purification & Purity:
Protein A affinity purified

Format:
Recombinant Rabbit Monoclonal Antibody. 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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