SMAD5 (C-term (clone 4B10-B10-B6), anti-human

Background:
SMAD5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. It is a receptor-regulated SMAD (R-SMAD), and acts as an intracellular signal transducer for the transforming growth factor beta superfamily. SMAD5 is activated through serine phosphorylation by BMP (bone morphogenetic proteins) type 1 receptor kinase. It is cytoplasmic in the absence of its ligand and migrates into the nucleus upon phosphorylation and complex formation with SMAD4. Here the SMAD5/SMAD4 complex stimulates the transcription of target genes.

Synonyms: DWFC, JV5-1, MADH5

Cellular Location: Cytoplasm Nucleus

Immunogen:
Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.

Gene Symbol: SMAD5(C-term)

Gene ID: 4090

Swiss prot: Q99717

Calculated MW: 60kDa

Recommended dilution:
WB 1:1000, ICC 1:75, FCM 1:100

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.