Sox2 (clone M551), anti-human, mouse, rat, chicken, ferret

Background:
Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation.

Background References

Boyer, L.A. et al. (2005) Cell. 122:947.
Loh, Y.H. et al. (2006) Nat Genet. 38:431.
Jeong, C.H. et al. (2010) Stem Cells. 28(12):2141.
Fang, L.. et al. (2014) Mol Cell. 55(4):537.

Immunogen: Clone M551 was generated from a recombinant protein corresponding to the full length sequence from human Sox2. This sequence is highly conserved in rat and mouse Sox2.

Specificity: The antibody was purified by Protein G chromatography. This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, and detects a full length recombinant human Sox2 protein

Buffer/Storage:
Mouse monoclonal purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.