Sp1 (phospho-T453) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4363
Background:
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.
Alternative Name:
Transcription factor Sp1, SP1, TSFP1
Application Dilution: IHC: 1:50~1:200
Specificity: p-Sp1 (T453) polyclonal antibody detects endogenous levels of Sp1 protein only when phosphorylated at Thr453
Immunogen:
Synthetic phosphopeptide derived from human Sp1 around the phosphorylation site of Threonine 453.
MW: ~ 90 kDa
Swis Prot.: P08047
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.
Alternative Name:
Transcription factor Sp1, SP1, TSFP1
Application Dilution: IHC: 1:50~1:200
Specificity: p-Sp1 (T453) polyclonal antibody detects endogenous levels of Sp1 protein only when phosphorylated at Thr453
Immunogen:
Synthetic phosphopeptide derived from human Sp1 around the phosphorylation site of Threonine 453.
MW: ~ 90 kDa
Swis Prot.: P08047
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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