SPARC polyclonal, anti-human, mouse, rat
€388.00
In stock
SKU
BS6738
Background:
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Alternative Name:
SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Application Dilution: WB: 1:500~1:2000, IHC: 1:50~1:200
Specificity: SPARC polyclonal antibody detects endogenous levels of SPARC protein.
Immunogen:
Recombinant full length Human SPARC.
MW: ~ 35 kDa
Swis Prot.: P09486
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Alternative Name:
SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Application Dilution: WB: 1:500~1:2000, IHC: 1:50~1:200
Specificity: SPARC polyclonal antibody detects endogenous levels of SPARC protein.
Immunogen:
Recombinant full length Human SPARC.
MW: ~ 35 kDa
Swis Prot.: P09486
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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